Composite

Part:BBa_K1797003:Design

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)


Blue Light Sensing System with GFP and RFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1338
    Illegal NgoMIV site found at 1410
    Illegal NgoMIV site found at 1500
    Illegal NgoMIV site found at 1518
    Illegal NgoMIV site found at 2030
    Illegal NgoMIV site found at 2323
    Illegal NgoMIV site found at 2417
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
    Illegal AgeI site found at 1052
    Illegal AgeI site found at 2198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2087
    Illegal BsaI.rc site found at 951


Design Notes

No.


Source

Blue-light-sensitive LOV domain in the light sensor YF1 is cloned from a protein called YtvA from B. subtilis. HK domain in the light sensor YF1 and the response regulator FixJ are both cloned from a protein called FixL from B. japonicum.

References